PH NBS

The PH NBS/PH NBS-D kits are supplied with 26 internal standards and controls for the measurement of carnitines and amino acids. Therefore, the PH NBS/PH NBS-D kits have capability of measuring 13 amino acids and 31 carnitine species, because of acylcarnitines with same chain length have similar performance characteristics. In this way, C16 internal standard can be used to determine the concentrations of the acylcarnitine series C16, C16:1, C16OH and C16:1OH. Additionally, unlabeled C16 can be used as an external control for all C16 acylcarnitine series. The analysis is done semi-quantitatively from dried blood spots and sample is spiked using the internal standard, the analytes are extracted from the dried blood matrix and analyzed with and without derivatization step using a tandem mass spectrometry system. The response of each analyte relative to their corresponding stable-isotope labeled internal standard is proportional to analyte concentration.